Last updated: 04/16/2026 14:40:15
A study of the safety of and immune response to varying doses of a vaccine against COVID-19 in healthy adults
EudraCT ID
Not applicable
EU CT Number
Not applicable
Trial status
Completed
Completed
Trial overview
Official title: A Phase I, First-Time-in Human (FTiH), Open-label, Dose Escalation, Non-randomized Study to Assess Safety, Reactogenicity and Immune Response of a CoV-2 SAM (LNP) Vaccine when Administered Intramuscularly on a 0, 1 Month Schedule in Healthy Adults 18 to 50 Years of Age
Trial description: The purpose of this study is to assess the safety, reactogenicity and immune response of a self-amplifying mRNA (SAM) lipid nanoparticle (LNP) platform with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike antigen (CoV-2 SAM [LNP] vaccine) in ascending doses when administered intramuscularly (IM) on a 0,1-month schedule to healthy adults 18 to 50 years of age. There will be no administration of escalated doses of the study vaccine.
Primary purpose:
Prevention
Trial design:
Single Group Assignment
Masking:
None (Open Label)
Allocation:
Not applicable
Primary outcomes:
Number of participants with solicited administration site adverse events after each vaccination
Timeframe: Within 7 days post each vaccination (vaccination at Day 1 and Day 31)
Number of participants with solicited systemic adverse events after each vaccination
Timeframe: Within 7 days post each vaccination (vaccination at Day 1 and Day 31)
Number of participants with unsolicited adverse events (AEs) after each vaccination
Timeframe: Within 30 days post each vaccination (vaccination at Day 1 and Day 31)
Number of participants with hematological and biochemical laboratory abnormalities at Day 2 compared with baseline (Day 1)
Timeframe: Day 2 compared to baseline (Day 1)
Number of participants with hematological and biochemical laboratory abnormalities at Day 8 compared with baseline (Day 1)
Timeframe: Day 8 compared to baseline (Day 1)
Number of participants with hematological and biochemical laboratory abnormalities at Day 31 compared with baseline (Day 31)
Timeframe: Day 31 compared to baseline (Day 1)
Number of participants with hematological and biochemical laboratory abnormalities at Day 32 compared with baseline (Day 1)
Timeframe: Day 32 compared to baseline (Day 1)
Number of participants with hematological and biochemical laboratory abnormalities at Day 38 compared with baseline (Day 1)
Timeframe: Day 38 compared to baseline (Day 1)
Number of participants with Medically-attended AEs (MAEs) from Day 1 to Day 61
Timeframe: Day 1 to Day 61
Number of participants with serious AEs (SAEs) from Day 1 to Day 61
Timeframe: Day 1 to Day 61
Number of participants with Adverse events of Special Interest (AESIs) [potential immune-mediated diseases (pIMDs) and COVID-19 cases) from Day 1 to Day 61
Timeframe: Day 1 to Day 61
Secondary outcomes:
Number of participants with MAEs, SAEs and AESIs (pIMDs and COVID-19 cases) from Day 1 up to Day 391
Timeframe: Day 1 to Day 391
Percentage of participants with anti-Spike (S) immunoglobulin G (IgG) antibody concentrations at Day 1, Day 31 and Day 61
Timeframe: At pre-vaccination on Day 1 and Day 31, and at Day 61
Number of participants with anti-Nucleocapsid (N) IgG antibody concentrations at Day 1, Day 31 and Day 61
Timeframe: At pre-vaccination on Day 1 and Day 31, and at Day 61
SARS-CoV-2 neutralizing antibody titers by Live Wild‑Type Virus Neutralization at Day 1, Day 31 and Day 61
Timeframe: At pre-vaccination on Day 1 and Day 31, and at Day 61
Percentage of participants with anti-S IgG antibody concentrations at Day 8, Day 15, Day 38 and Day 45
Timeframe: At Day 8, Day 15, Day 38 and Day 45
Number of participants with anti-N IgG antibody concentrations at Day 8, Day 15, Day 38 and Day 45
Timeframe: Day 8, Day 15, Day 38 and Day 45
Cell mediated immune (CMI) response in terms of frequency of SARS-CoV-2 Spike-specific Cluster of Differentiation (CD) 4+ T-cells secreting at least 2 markers including one cytokine
Timeframe: Pre-vaccination on Day 1 and Day 31, and at Day 61
CMI response in terms of frequency of SARS-CoV-2 Spike-specific CD 8+ T-cells secreting at least 2 markers including one cytokine
Timeframe: Pre-vaccination on Day 1 and Day 31, and at Day 61
Interventions:
Biological/vaccine: 1 µg CoV2 SAM (LNP)
Enrollment:
10
Observational study model:
Not applicable
Primary completion date:
2021-04-06
Time perspective:
Not applicable
Clinical publications:
Not applicable
Medical condition
Virus Diseases
Product
Not applicable
Collaborators
Not applicable
Study date(s)
February 2021 to April 2022
Type
Interventional
Phase
1
Participation criteria
Sex
Female & Male
Age
18 - 50 Years
Accepts healthy volunteers
Yes
- Participants who, in the opinion of the investigator, can and will comply with the requirements of the protocol.
- Written or thumb printed informed consent obtained from the participant prior to performance of any study specific procedure.
- Medical conditions
- Individuals with signs and symptoms consistent with COVID-19, according to CDC guidelines and following clinical judgement.
Inclusion and exclusion criteria
Inclusion criteria:
- Participants who, in the opinion of the investigator, can and will comply with the requirements of the protocol.
- Written or thumb printed informed consent obtained from the participant prior to performance of any study specific procedure.
- Healthy participants as established by medical history and clinical examination before entering into the study.
- A male or female between, and including, 18 and 50 years of age at the time of first study intervention administration.
- Body Mass Index>18 Kg/m^2 and <30 Kg/m^2.
- Participants with following hematological/biochemical parameters:
- White Blood Cells within the study designated laboratory normal range. Participants with FDA toxicity grade 1 differential cell counts and considered not clinically significant may be enrolled at the discretion of the investigator, and with the review and approval of the medical monitor.
- Platelets = 125,000 – 500,000 cells/mm^3.
- Hemoglobin within normal range of the study designated laboratory.
- Alanine aminotransferase within the study designated laboratory normal range.
- Aspartate aminotransferase within the study designated laboratory normal range Total bilirubin within the study designated laboratory normal range.
- Alkaline phosphatase within the study designated laboratory normal range.
- Blood urea nitrogen within the study designated laboratory normal range.
- Serum creatinine less than or equal to 1.1 times study designated laboratory’s upper limit of normal.
- Seronegative for hepatitis B surface antigen, hepatitis C virus antibodies, or human immunodeficiency virus antibodies.
- Female participants of non-childbearing potential may be enrolled in the study. Non-childbearing potential is defined as pre-menarche, current bilateral tubal ligation or occlusion, hysterectomy, bilateral ovariectomy or post-menopause.
- Female participants of childbearing potential may be enrolled in the study, if the participant:
- has practiced adequate contraception for 1 month prior to study intervention administration, and,
- has a negative pregnancy test on the day of study intervention administration, and,
- has agreed to continue adequate contraception during the entire treatment period and for 2 months after completion of the study intervention administration series.
Exclusion criteria:
- Medical conditions
- Individuals with signs and symptoms consistent with COVID-19, according to CDC guidelines and following clinical judgement.
- Nasal and/or oral swab positive for SARS-CoV-2 by RT-PCR within the last 30 days, unless participant has had a subsequent negative swab and is asymptomatic.
- Close contact (within 30 days prior to study intervention administration) with anyone known to have SARS-CoV-2 infection.
- Individuals currently working in occupations with high risk of exposure to SARS-CoV-2 (e.g., healthcare workers, emergency response personnel).
- Any confirmed or suspected immunosuppressive/immunodeficient condition based on medical history and physical examination.
- Family history of congenital/hereditary immunodeficiency.
- History of or current autoimmune disease.
- History of any reaction/hypersensitivity likely to be exacerbated by any components of the study intervention.
- History of hypersensitivity/severe allergic reaction to any previous licensed/unlicensed vaccine.
- Lymphoproliferative disorder/malignancy within previous 5 years (excluding effectively treated non-melanotic skin cancer).
- History of recurrent anemia within the last 6 months.
- Hypersensitivity to latex.
- Any acute/chronic, clinically significant disease The following conditions will be exclusionary:
- Diabetes mellitus (type I or II), with exception of gestational diabetes.
- Respiratory disease such as:
- Chronic Pulmonary diseases,
- Bronchopulmonary dysplasia,
- Uncontrolled asthma/asthma necessitating treatment with chronic systemic/inhaled glucocorticoids.
- Significant and/or uncontrolled psychiatric illness:
- hospitalization for psychiatric illness, history of suicide attempts/confinement for danger to self/others within 5 years,
- clinically significant depression.
- Major neurological disease including:
- seizure or adulthood epilepsy (note: history of febrile convulsion in childhood is not exclusionary),
- myasthenia gravis,
- history of repetitive migraine mal/status migrainosus.
- Significant cardiovascular disease, including:
- Uncontrolled arterial hypertension,
- Congenital heart disease,
- Previous myocardial infarction,
- Valvular heart disease or history of rheumatic fever,
- Previous bacterial endocarditis,
- History of cardiac surgery,
- Personal/ family history of cardiomyopathy/sudden adult death.
- Asplenia, functional asplenia/any condition resulting in the absence/removal of the spleen.
- Hereditary angioedema, acquired angioedema/ idiopathic forms of angioedema.
- History of, or concurrent, autoimmune thyroid disease regardless of treatment and thyroid status as well as any uncontrolled thyroid disease.
- Idiopathic urticaria within the past year.
- Any other significant uncontrolled medical illness within 3 months prior to study intervention administration.
- Acute illness and/or fever at the time of screening. Participants with acute illness and/ or fever at the time of screening may be re screened later.
- Participants with a minor illness without fever may be enrolled.
- Any other clinical condition that might pose additional risk to the participant due to participation in the study. Prior/Concomitant therapy
- Use of any investigational or non-registered product other than the study intervention(s) during the period beginning 45 days before the first dose (Day -45 to Day 1), or their planned use during the study.
- Planned administration/administration of a vaccine not foreseen by the study protocol in the period starting 30 days before the vaccination and ending 30 days after, with the exception of any licensed influenza vaccine which may be administered >15 days before/after vaccination.
- Planned administration/administration of an Emergency Use Authorization vaccine against SARS-CoV-2 in the period starting prior to the first dose of study intervention and ending 15 days after the second dose of study intervention
- Administration of long-acting immune-modifying drugs at any time during the study
- Administration of immunoglobulins and/or any blood products or plasma derivatives during the period starting 3 months before the administration of the first dose until study end.
- Chronic administration of immunosuppressants/other immune-modifying drugs during the period starting 6 months prior to the first dose. For corticosteroids, this will mean prednisone equivalent >10 mg/day. Topical steroids are allowed. Prior/Concurrent clinical study experience
- Receipt of any other SARS-CoV-2 or other experimental coronavirus vaccine at any time prior to or during the study
- Concurrently participating in another clinical study, at any time during the study period, in which the participant has been/will be exposed to an investigational/a non-investigational intervention Other exclusions
- Pregnant/lactating female
- Female planning to become pregnant/planning to discontinue contraceptive precautions
- Alcohol and/or drug abuse.
- Current/history of chronic tobacco/marijuana smoking/vaping.
- Participants with extensive tattoos covering deltoid region on both the arms that would preclude the assessment of local reactogenicity.
Trial location(s)
Location
GSK Investigational Site
Rochester, New York, United States, 14609
Status
Study Complete
Study documents
Protocol
Available language(s): English
Statistical analysis plan
Available language(s): English
If you wish to request for full study report, please contact - [email protected]
Results overview
Results posted on ClinicalTrials.gov
Recruitment status
Completed
Actual primary completion date
2021-04-06
Actual study completion date
2022-19-04
Plain language summaries
Plain language summaries of clinical trial results for Phase 2-4 clinical trials that were initiated on or after January 2022 will be posted by GSK within one year following study completion.
Additional information about the trial
Additional information
Not applicable
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